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The peak fractions were concentrated on a 50 kDa MWCO Amicon concentrator and applied onto a Superdex 200 10/300 column (Cytiva) pre-equilibrated in SEC buffer (20 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 1 mM TCEP)

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17 : 18591867 Isner JM, Kearney M, Bortman S and Passeri J (1995) Apoptosis in human atherosclerosis and restenosis

The ratio of Bacteroidetes to Firmicutes in your system is a biomarker for obesity